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InVitria Blog | Cell Culture and Biotech Manufacturing Insights

Expansion of Adherent HEK293t Cells in Flasks in Chemically Defined Cell Culture Media

Posted By Attie Pennybaker on 11/18/19 4:34 PM

Workflow Summary:

New call-to-actionCombine supplements of OptiPEAK HEK293t media. Generate stock solutions and prepare reagents. Adapt HEK293t cells in log phase growth from serum to OptiPEAK HEK293t chemically defined, serum free media. Subculture HEK293t cells every 2-3 days in T-75 flasks, calculating doubling time and population doublings to determine cumulative expansion results.

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Stabilizing Virus with Albumin to Improve Yield

Posted By Randy Alfano Ph.D. on 10/22/19 11:02 AM

Workflow Summary:

Expand virus-producing cells to desired confluency. RemoveNew call-to-action growth medium and add the desired virus to be expanded (virus or plasmid DNA). Add Exbumin (Product Number: 777HSA097S) to the expression media and allow virus to propagate. Harvest culture and determine virus titer by preferred method.

Here, a general protocol is described for the addition of recombinant albumin, in the form of the product Exbumin, to virus production systems to stabilize infectious particles and thereby maximize viral yield.

 

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Recombinant Albumin for Stabilization of Live-Attenuated Viral Vaccines

Posted By Jeanne McAdara Ph.D. on 4/16/19 2:00 PM

Enhanced liquid-phase stability of live-attenuated Flavivirus vaccines using recombinant albumin

The single-stranded RNA viruses belonging to the genus Flavivirus (family, Flaviviridae) are significant to global public health due to the high rates of morbidity and mortality they cause.1 For example, Yellow Fever sickens approximately 200,000 people and kills 30,000 annually.2 Other viruses belonging to this genus include the mosquito-borne diseases Dengue Fever, Japanese encephalitis, West Nile virus, and Zika virus, and the tick-borne diseases Tick-borne Encephalitis, Kyasanur Forest Disease, Alkhurma disease, and Omsk hemorrhagic fever.

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