Combine kit supplements of OptiVERO. Prepare complementary reagents. Wash cells to remove traces of FBS and harvest from growth surface. Seed in OptiVERO and grow to confluence. Add desired virus to be expanded in minimal basal EMEM. Incubate for 2 hours at 37°C. Add fresh OptiVERO. Allow virus expansion for 3-13 days. Quantify virus by desired method.
Posted By Annie Cunningham on 3/19/19 11:36 AM
Posted By Attie Pennybaker on 3/4/19 12:30 PM
Supplement basal media with ITSE + A to a final 1x-2x concentration. Reduce typical percentage of added FBS by 50-90%. Expand cells as normal in ITSE + A supplemented media for desired expansion time. Perform post-expansion cell count and doubling-time calculation.
Posted By Annie Cunningham on 2/27/19 2:02 PM
Combine supplements of OptiVERO Serum Free Media (SFM). Prepare complementary reagents. Wash cells to remove traces of donor serum. Remove cells from growth surface. Wash and collect cells. Centrifuge. Resuspend. Count. Seed in OptiVERO SFM.
Posted By Annie Cunningham on 2/18/19 8:41 AM
Combine supplements of OptiPEAK T Lymphocyte media. Isolate human T lymphocytes from whole blood. Wash to remove traces of donor serum. Activate and expand T lymphocytes (7-10 days) in OptiPEAK T Lymphocyte serum free media. Harvest T lymphocytes. Perform post-expansion, flow cytometric analysis of phenotypic surface markers.