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InVitria Blog | Cell Culture and Biotech Manufacturing Insights

Stabilizing Virus with Albumin to Improve Yield

Posted By Randy Alfano Ph.D. on 4/11/19 11:50 AM

Cellastim S Application Note #1 Download CTA ButtonWorkflow Summary:

Expand virus-producing cells to desired confluency. Remove growth medium and add the desired virus to be expanded (virus or plasmid DNA). Add Cellastim S (Product Number: 777HSA017S) to the expression media and allow virus to propagate. Harvest culture and determine virus titer by preferred method.

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Virus Production Using VERO Cells with Microcarriers Without Serum in Chemically Defined Cell Culture Media

Posted By Randy Alfano Ph.D. on 4/2/19 11:30 AM

OptiVERO App Note #3 Download CTA ButtonWorkflow Summary:

Combine supplements of OptiVEROPrepare complementary reagents. Wash cells to remove traces of FBS and harvest from growth surface. Seed in OptiVERO and expand in cell culture flasks for at least 3 passages to allow for adaptation. Prepare microcarrier system, inoculate with VERO cells, and incubate 3-4 days for adhesion and expansion. Add virus of interest in minimal basal MEM. Incubate for 2 hours at 37°C. Add fresh OptiVERO. Allow virus expansion for 3-13 days. Quantify virus by desired method.

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Virus Production Using VERO Cells in Flasks without Serum in Chemically Defined Cell Culture Media

Posted By Annie Cunningham on 3/19/19 11:36 AM

OptiVERO App Note #1 Download CTA ButtonWorkflow Summary:

Combine kit supplements of OptiVERO. Prepare complementary reagents. Wash cells to remove traces of FBS and harvest from growth surface. Seed in OptiVERO and grow to confluence. Add desired virus to be expanded in minimal basal EMEM. Incubate for 2 hours at 37°C. Add fresh OptiVERO. Allow virus expansion for 3-13 days. Quantify virus by desired method. 

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Reducing Serum-Associated Contaminants from Growth Media in Primary Cell Culture

Posted By Attie Pennybaker on 3/4/19 12:30 PM

ITSE App Note #1 Download CTA ButtonWorkflow Summary:

Supplement basal media with ITSE + A to a final 1x-2x concentration. Reduce typical percentage of added FBS by 50-90%. Expand cells as normal in ITSE + A supplemented media for desired expansion time. Perform post-expansion cell count and doubling-time calculation.

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Expansion of VERO Cells Without Serum in Chemically Defined Cell Culture Media

Posted By Annie Cunningham on 2/27/19 2:02 PM

OptiVERO App Note #1 Download CTA ButtonWorkflow Summary:

Combine supplements of OptiVERO Serum Free Media (SFM). Prepare complementary reagents. Wash cells to remove traces of donor serum. Remove cells from growth surface. Wash and collect cells. Centrifuge. Resuspend. Count. Seed in OptiVERO SFM. 

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