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InVitria Blog | Cell Culture and Biotech Manufacturing Insights

ASGCT 2019: Remarkable Milestones and Achievements in Cell and Gene Therapy

Posted By Attie Pennybaker on 5/7/19 9:00 AM

The American Society of Gene & Cell Therapy (ASGCT) held their 22nd annual meeting in Washington D.C. last week from April 29th through May 2nd bringing together a record number of over 3,400 attendees to share and discuss the remarkable work going on to advance this field. With 23 Scientific Symposia and 1000 abstracts presented through talks and poster sessions over the course of the conference, there was no shortage of knowledge to be shared.

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Recombinant Albumin for Stabilization of Live-Attenuated Viral Vaccines

Posted By Jeanne McAdara Ph.D. on 4/16/19 2:00 PM

Enhanced liquid-phase stability of live-attenuated Flavivirus vaccines using recombinant albumin

The single-stranded RNA viruses belonging to the genus Flavivirus (family, Flaviviridae) are significant to global public health due to the high rates of morbidity and mortality they cause.1 For example, Yellow Fever sickens approximately 200,000 people and kills 30,000 annually.2 Other viruses belonging to this genus include the mosquito-borne diseases Dengue Fever, Japanese encephalitis, West Nile virus, and Zika virus, and the tick-borne diseases Tick-borne Encephalitis, Kyasanur Forest Disease, Alkhurma disease, and Omsk hemorrhagic fever.

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Stabilizing Virus with Albumin to Improve Yield

Posted By Randy Alfano Ph.D. on 4/11/19 11:50 AM

Cellastim S Application Note #1 Download CTA ButtonWorkflow Summary:

Expand virus-producing cells to desired confluency. Remove growth medium and add the desired virus to be expanded (virus or plasmid DNA). Add Cellastim S (Product Number: 777HSA017S) to the expression media and allow virus to propagate. Harvest culture and determine virus titer by preferred method.

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Virus Production Using VERO Cells with Microcarriers Without Serum in Chemically Defined Cell Culture Media

Posted By Randy Alfano Ph.D. on 4/2/19 11:30 AM

OptiVERO App Note #3 Download CTA ButtonWorkflow Summary:

Combine supplements of OptiVEROPrepare complementary reagents. Wash cells to remove traces of FBS and harvest from growth surface. Seed in OptiVERO and expand in cell culture flasks for at least 3 passages to allow for adaptation. Prepare microcarrier system, inoculate with VERO cells, and incubate 3-4 days for adhesion and expansion. Add virus of interest in minimal basal MEM. Incubate for 2 hours at 37°C. Add fresh OptiVERO. Allow virus expansion for 3-13 days. Quantify virus by desired method.

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Virus Production Using VERO Cells in Flasks without Serum in Chemically Defined Cell Culture Media

Posted By Annie Cunningham on 3/19/19 11:36 AM

OptiVERO App Note #1 Download CTA ButtonWorkflow Summary:

Combine kit supplements of OptiVERO. Prepare complementary reagents. Wash cells to remove traces of FBS and harvest from growth surface. Seed in OptiVERO and grow to confluence. Add desired virus to be expanded in minimal basal EMEM. Incubate for 2 hours at 37°C. Add fresh OptiVERO. Allow virus expansion for 3-13 days. Quantify virus by desired method. 

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