Recently I had the opportunity to attend the Treg Summit in Boston to listen to the ground-breaking clinical and preclinical research in re-balancing the immune system in the context of autoimmunity and organ transplant via the use of polyclonal or antigen-induced T regulatory cells. Overall, this cell therapy holds immense promise as evidenced by both preclinical models and early clinical evaluations.
Virus production is an important application of cell culture systems, both for vaccine manufacturing as well as for gene therapy. During the cell culture process, the infectious particles produced are subjected to several physical stresses inherent to the cell culture system that may cause inactivation and reduce the overall productivity of the culture.Here, a general protocol is described for the addition of recombinant albumin, in the form of the product Cellastim S, to virus production systems to stabilize infectious particles and thereby maximize viral yield.
Virus Production Using VERO Cells with Microcarriers Without Serum in Chemically Defined Cell Culture Media
Posted By Randy Alfano Ph.D. on 4/2/19 11:30 AM
Combine supplements of OptiVERO. Prepare complementary reagents. Wash cells to remove traces of FBS and harvest from growth surface. Seed in OptiVERO and expand in cell culture flasks for at least 3 passages to allow for adaptation. Prepare microcarrier system, inoculate with VERO cells, and incubate 3-4 days for adhesion and expansion. Add virus of interest in minimal basal MEM. Incubate for 2 hours at 37°C. Add fresh OptiVERO. Allow virus expansion for 3-13 days. Quantify virus by desired method.
Posted By Randy Alfano Ph.D. on 2/4/19 8:36 AM
January 27th - 31st InVitria will be in California for Engineering Conferences International: Advancing Manufacturing Cell & Gene Therapy VI. This unique conference will focus on the technological challenges and engineering solutions in the cell and gene therapy field. Limited to 400 participants, this conference is an opportunity for key academic and industrial leaders in the space to showcase and discuss new and existing approaches.