Expand virus-producing cells to desired confluency. Remove growth medium and add the desired virus to be expanded (virus or plasmid DNA). Add Cellastim S (Product Number: 777HSA017S) to the expression media and allow virus to propagate. Harvest culture and determine virus titer by preferred method.
Virus Production Using VERO Cells with Microcarriers Without Serum in Chemically Defined Cell Culture Media
Posted By Randy Alfano Ph.D. on 4/2/19 11:30 AM
Combine supplements of OptiVERO. Prepare complementary reagents. Wash cells to remove traces of FBS and harvest from growth surface. Seed in OptiVERO and expand in cell culture flasks for at least 3 passages to allow for adaptation. Prepare microcarrier system, inoculate with VERO cells, and incubate 3-4 days for adhesion and expansion. Add virus of interest in minimal basal MEM. Incubate for 2 hours at 37°C. Add fresh OptiVERO. Allow virus expansion for 3-13 days. Quantify virus by desired method.
Posted By Randy Alfano Ph.D. on 2/4/19 8:36 AM
January 27th - 31st InVitria will be in California for Engineering Conferences International: Advancing Manufacturing Cell & Gene Therapy VI. This unique conference will focus on the technological challenges and engineering solutions in the cell and gene therapy field. Limited to 400 participants, this conference is an opportunity for key academic and industrial leaders in the space to showcase and discuss new and existing approaches.
Posted By Randy Alfano Ph.D. on 9/10/18 9:48 AM
We are pleased to announce that on Tuesday, September 18th InVitria will be hosting a webinar in partnership with Genetic Engineering & Biotechnology News!
Here is the webinar abstract:
The advent of recombinant antibody production in mammalian cell lines has revolutionized modern medicine. Clinical success of these agents has challenged the underlying biological manufacturing systems to produce quantities sufficient to supply the ever-expanding commercial markets.