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InVitria Blog | Cell Culture and Biotech Manufacturing Insights

Randy Alfano Ph.D.

Randy Alfano Ph.D.
Dr. Alfano leads InVitria’s product development team, where he utilizes his expertise in cell biology to optimize cell culture media formulations and design cell culture systems used in cell therapy, gene therapy, vaccines, and regenerative medicine.
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Recent Posts

Stabilizing Virus with Albumin to Improve Yield

Posted By Randy Alfano Ph.D. on 4/11/19 11:50 AM

Cellastim S Application Note #1 Download CTA ButtonWorkflow Summary:

Expand virus-producing cells to desired confluency. Remove growth medium and add the desired virus to be expanded (virus or plasmid DNA). Add Cellastim S (Product Number: 777HSA017S) to the expression media and allow virus to propagate. Harvest culture and determine virus titer by preferred method.

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Virus Production Using VERO Cells with Microcarriers Without Serum in Chemically Defined Cell Culture Media

Posted By Randy Alfano Ph.D. on 4/2/19 11:30 AM

OptiVERO App Note #3 Download CTA ButtonWorkflow Summary:

Combine supplements of OptiVEROPrepare complementary reagents. Wash cells to remove traces of FBS and harvest from growth surface. Seed in OptiVERO and expand in cell culture flasks for at least 3 passages to allow for adaptation. Prepare microcarrier system, inoculate with VERO cells, and incubate 3-4 days for adhesion and expansion. Add virus of interest in minimal basal MEM. Incubate for 2 hours at 37°C. Add fresh OptiVERO. Allow virus expansion for 3-13 days. Quantify virus by desired method.

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ECI Conference 2019: The Latest Innovations and Trends in Cell & Gene Therapy

Posted By Randy Alfano Ph.D. on 2/4/19 8:36 AM

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Join InVitria at ECI 2019

Posted By Randy Alfano Ph.D. on 1/28/19 9:53 AM

January 27th - 31st InVitria will be in California for Engineering Conferences International: Advancing Manufacturing Cell & Gene Therapy VI. This unique conference will focus on the technological challenges and engineering solutions in the cell and gene therapy field. Limited to 400 participants, this conference is an opportunity for key academic and industrial leaders in the space to showcase and discuss new and existing approaches.  

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WEBINAR: The Cell Culture Revolution: Commercializing Therapeutic Cell Systems

Posted By Randy Alfano Ph.D. on 9/10/18 9:48 AM

join the cell culture revolution webinar!

We are pleased to announce that on Tuesday, September 18th InVitria will be hosting a webinar in partnership with Genetic Engineering & Biotechnology News!

Here is the webinar abstract:

The advent of recombinant antibody production in mammalian cell lines has revolutionized modern medicine. Clinical success of these agents has challenged the underlying biological manufacturing systems to produce quantities sufficient to supply the ever-expanding commercial markets. 

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