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InVitria Blog | Cell Culture and Biotech Manufacturing Insights

Randy Alfano

Randy Alfano
Dr. Alfano joined InVitria in 2012 and currently leads the product development team, where he utilizes his expertise in media design and optimization for biomanufacturing and stem/primary cells. Prior to joining the company, Dr. Alfano was the Process Development Manager for XBiotech in Austin, TX where he specialized in the development and optimization of the company’s cell culture processes in mammalian cells for cancer biologics. Previously, he was promoted from Postdoctoral Fellow to Senior Scientist at the Cancer Research Institute of Scott and White Hospital where he developed in vivo animal models for metastatic prostate cancer and was instrumental in developing thorough in vitro and in vivo models for immunostimulatory antigens. Dr. Alfano received his Ph.D. from Texas A&M Health Science Center College of Medicine.
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Recent Posts

WEBINAR: The Cell Culture Revolution: Commercializing Therapeutic Cell Systems

Posted By Randy Alfano on 9/10/18 9:48 AM

join the cell culture revolution webinar!

We are pleased to announce that on Tuesday, September 18th InVitria will be hosting a webinar in partnership with Genetic Engineering & Biotechnology News!

Here is the webinar abstract:

The advent of recombinant antibody production in mammalian cell lines has revolutionized modern medicine. Clinical success of these agents has challenged the underlying biological manufacturing systems to produce quantities sufficient to supply the ever-expanding commercial markets. 

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InVitria at the American Association of Immunologists 2018 Conference

Posted By Randy Alfano on 5/18/18 9:59 AM


This year at the American Association of Immunologists 2018 Conference in Austin, Texas, I had the pleasure of meeting some of the field’s brightest scientists deep in my home town. While enjoying only a small sample of blues, rock, country music and some Texas-authentic Tex-Mex that the live music capital of the world has to offer, I was able to catch up on some of the latest developments in the field, network with colleagues, and speak with scientists presenting their latest research. 

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Transferrin: An Iron Transport Protein - Recombinant Human Transferrin in Cell Culture Media

Posted By Randy Alfano on 5/16/18 11:17 AM

Transferrin is an iron binding protein found in blood plasma that is critical to cell health. Transferrin supplies iron to cells naturally in the body and as a supplement in cell culture media.


Transferrin Function and Structure

Human serum transferrin, a bilobal ~75 kD glycoprotein that has the ability to reversibly bind Fe3+ with nanomolar affinity, represents one of the major vehicles for iron delivery to cells both in vivo and in vitro3. Produced in the liver, transferrin is found in the blood plasma as a heterogeneous population at approximately 2.5 mg/mL consisting of diferric (holo or iron-saturated), monoferric N-lobe, monoferric C-lobe (partially iron saturated) or apo transferrin4.

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