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InVitria Blog | Cell Culture and Biotech Manufacturing Insights

Attie Pennybaker

Attie Pennybaker
Atherly Pennybaker is a Product Applications Scientist with InVitria. At InVitria she enjoys leveraging her scientific background to help customers successfully transition to blood-free, chemically defined systems. Prior to InVitria, she received a Bachelor of Science in Chemical and Biological Engineering from Colorado State University. During her time there, she conducted undergraduate research in tissue engineering where she co-authored a paper on the osteoinductivity of an engineered biomimetic periosteum. She also worked on the design of a natural cell and tissue scaffold by means of electrospinning demineralized bone matrix.
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Recent Posts

Expansion of Adherent HEK293t Cells in Flasks in Chemically Defined Cell Culture Media

Posted By Attie Pennybaker on 11/18/19 4:34 PM

Workflow Summary:

New call-to-actionCombine supplements of OptiPEAK HEK293t media. Generate stock solutions and prepare reagents. Adapt HEK293t cells in log phase growth from serum to OptiPEAK HEK293t chemically defined, serum free media. Subculture HEK293t cells every 2-3 days in T-75 flasks, calculating doubling time and population doublings to determine cumulative expansion results.

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ASGCT 2019: Remarkable Milestones and Achievements in Cell and Gene Therapy

Posted By Attie Pennybaker on 5/7/19 9:00 AM

The American Society of Gene & Cell Therapy (ASGCT) held their 22nd annual meeting in Washington D.C. last week from April 29th through May 2nd bringing together a record number of over 3,400 attendees to share and discuss the remarkable work going on to advance this field. With 23 Scientific Symposia and 1000 abstracts presented through talks and poster sessions over the course of the conference, there was no shortage of knowledge to be shared.

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Reducing Serum-Associated Contaminants from Growth Media in Primary Cell Culture

Posted By Attie Pennybaker on 3/4/19 12:30 PM

ITSE App Note #1 Download CTA ButtonWorkflow Summary:

Supplement basal media with ITSE + A to a final 1x-2x concentration. Reduce typical percentage of added FBS by 50-90%. Expand cells as normal in ITSE + A supplemented media for desired expansion time. Perform post-expansion cell count and doubling-time calculation.

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InVitria's Top Insights from ISCT 2018 - Montreal

Posted By Attie Pennybaker on 5/15/18 4:00 PM

The International Society of Cell & Gene Therapy (ISCT) has a rich history of bringing together leading professionals who are committed to advancing the field. This year, from May 2nd to May 5th, nearly 1500 of those leading professionals from across the globe gathered in the bustling Canadian city of Montreal to share the latest breakthroughs and innovations, partake in thought-provoking conversations, and create lasting connections at the ISCT 2018 annual conference. Although I did not get a chance to indulge in the famed Canadian poutine dish, I left the country full of something better: new ideas, deeper knowledge, and a greater appreciation for the potential implications and benefits of cell and gene therapy innovation.

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3 Takeaways from the World Vaccine Congress 2018

Posted By Attie Pennybaker on 4/11/18 10:16 AM

Innovation, technology, and success has driven the vaccine industry to remain at the forefront of biotechnology and this year’s World Vaccine Congress was no exception. Taking place April 2-5 in Washington D.C., the 18th annual World Vaccine Congress lured over 1000 attendees from more than 300 leading global institutions to deliver the largest establishment of this event yet. Being surrounded by such a vast amount of knowledge and key influencers, my experience representing InVitria here was one full of inspiration.

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