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InVitria Blog | Cell Culture and Biotech Manufacturing Insights

Annie Cunningham

Annie Cunningham
Annie Cunningham joined InVitria in 2018 as a Cell Culture Scientist. After graduating from Michigan State University, Annie began her career at Seattle Genetics developing potency assays for antibody-drug conjugates for cancer treatment. Annie shifted focus into immunotherapy and contributed to the development of the CAR T program at Juno Therapeutics. After moving to Colorado in 2017, Annie continued researching the human immune system as it relates to the gut microbiome at the University of Colorado. In her role at InVitria, she enjoys the new challenge of formulating completely blood-free, chemically defined cell culture media for the scalable and sustainable manufacturing of life-saving medicines.
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Recent Posts

Virus Production Using VERO Cells in Flasks without Serum in Chemically Defined Cell Culture Media

Posted By Annie Cunningham on 3/19/19 11:36 AM

Workflow SumOptiVERO App Note #1 Download CTA Buttonmary:

Combine kit supplements of OptiVERO. Prepare complementary reagents. Wash cells to remove traces of FBS and harvest from growth surface. Seed in OptiVERO and grow to confluence. Add desired virus to be expanded in minimal basal EMEM. Incubate for 2 hours at 37°C. Add fresh OptiVERO. Allow virus expansion for 3-13 days. Quantify virus by desired method. 

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Expansion of VERO Cells Without Serum in Chemically Defined Cell Culture Media

Posted By Annie Cunningham on 2/27/19 2:02 PM

OptiVERO App Note #1 Download CTA ButtonWorkflow Summary:

Combine supplements of OptiVERO Serum Free Media (SFM). Prepare complementary reagents. Wash cells to remove traces of donor serum. Remove cells from growth surface. Wash and collect cells. Centrifuge. Resuspend. Count. Seed in OptiVERO SFM. 

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Expansion of T Cells without Serum in Chemically Defined Media

Posted By Annie Cunningham on 2/18/19 8:41 AM

OptiPEAK Application Note Download CTAWorkflow Summary:

Combine supplements of OptiPEAK T Lymphocyte media. Isolate human T lymphocytes from whole blood. Wash to remove traces of donor serum. Activate and expand T lymphocytes (7-10 days) in OptiPEAK T Lymphocyte serum free media. Harvest T lymphocytes. Perform post-expansion, flow cytometric analysis of phenotypic surface markers.

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